Composite

Part:BBa_K988000

Designed by: Erin Kelly   Group: iGEM13_Lethbridge_Canada   (2013-06-21)


K314100_E.coli PELB signal sequence_OXT_His-tag_double stop codon_pSB1C3

We have a high efficiency constitutive promoter and RBS preceding the signal sequence for maximum production. We used the PELB signal sequence from E. coli to guide the protein into the periplasmic space. We added a histidine tag to the end of the Oxytocin-NeurophysinI gene. This protein is also known as prepro-oxyphysin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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