Coding

Part:BBa_K977000

Designed by: Eric Kessler   Group: iGEM13_BV_CAPS_Kansas   (2013-06-06)


Pyruvate Kinase derived from Rabbit Muscle

This part is the 1569 bp long coding sequence of a pyruvate kinase gene variant derived from rabbit muscle (Oryctolagus cuniculus pyruvate kinase, muscle (PKM), transcript variant 1, mRNA)

GenBank link - http://www.ncbi.nlm.nih.gov/nucleotide/307548865?report=genbank&log$=nuclalign&blast_rank=1&RID=V0YWBB4E01R

Pyruvate kinase is a key regulatory enzyme within glycolysis that removes a phosphate from phosphoenolpyruvate (PEP) adding it to ADP in the production of ATP and pyruvate. The enzyme is allosterically regulated by both inhibitory and excitatory substances.

We are attempting to use pyruvate kinase to enhance the downstream metabolic production of alkanes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 95
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 259


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