Composite

Part:BBa_K976010

Designed by: Amna Sohail   Group: iGEM13_MCIT_Indianapolis   (2013-06-21)


psbA2 promoter, 5'UTR, and RBS + Firefly Luciferase

The psbA2 promoter, 5'UTR, and RBS + Firefly Luciferase BioBrick represents a composite part. The psbA2 promoter, 5'UTR, and RBS portion is derived from regulatory BioBrick, BBa_K390017, which was designed by Cody Tramp of the 2010 Utah State Group. The firefly luciferase portion is derived from BBa_I712019, which was designed by Anja Korenčič of the 2007 Ljubljana Group. Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters. This part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 901


[edit]
Categories
Parameters
None