Coding

Part:BBa_K957015

Designed by: Dylan Patrick Webster   Group: iGEM12_Cornell   (2012-10-28)


Naphthalene Dioxygenase (From Pseudomonas NAH7 plasmid)

When the nah operon from the NAH7 Pseudomonas plasmid is heterologously expressed in E. coli, the cells have the ability to synthesize indigo from tryptophan [1].

E. coli contains the enzyme tryptophanase, which splits the indole group from the peptide backbone. Naphthalene dioxygenase, from the nah operon, then creates a compound which will spontaneously eliminate water and be air oxidized into indigo as the final product.



[1] Ensley, B., Ratzkin, B., Osslund, T., Simon, M., Wackett, L., & Gibson, D. (1983). Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo. Science, 222(4620), 167-9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3200
    Illegal XhoI site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1804
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2921
    Illegal BsaI.rc site found at 2755
    Illegal SapI.rc site found at 3285


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