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Part:BBa_K957012

Designed by: Steven Tiwen Chen & Dylan Patrick Webster   Group: iGEM12_Cornell   (2012-10-28)


Salicylate inducible polHis-MtrB

This part includes the mtrB coding sequence with a poly-His tag appended to the N-terminus.

The purpose of this part is to upregulate mtrB expression in response to salicylate. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to salicylate. If coupled to the expression of a BioBrick part that degrades naphthalene to salicylate, this part will function as part of a naphthalene biosensor.

Because of the His tag, this part may also be used for in vitro characterization of mtrB, and will also facilitate the purification of mtrB to obtain its crystal structure.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BamHI site found at 1073
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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