Part:BBa_K957005

Constitutive expression of nah Operon for degradation of naphthalene to salicylate

One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1910
  Illegal PstI site found at 5905
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 4165
  Illegal PstI site found at 1910
  Illegal PstI site found at 5905
  Illegal NotI site found at 8208
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 5258
  Illegal BamHI site found at 3243
  Illegal BamHI site found at 5945
  Illegal XhoI site found at 1354
  Illegal XhoI site found at 3742
  Illegal XhoI site found at 3894
  Illegal XhoI site found at 9396
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1910
  Illegal PstI site found at 5905
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1910
  Illegal PstI site found at 5905
  Illegal NgoMIV site found at 1847
  Illegal NgoMIV site found at 3622
  Illegal NgoMIV site found at 5496
  Illegal NgoMIV site found at 6108
  Illegal NgoMIV site found at 9421
  Illegal AgeI site found at 685
  Illegal AgeI site found at 3616
  Illegal AgeI site found at 6324
  Illegal AgeI site found at 8319
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2964
  Illegal BsaI site found at 3637
  Illegal BsaI site found at 4228
  Illegal BsaI site found at 5394
  Illegal BsaI.rc site found at 2798
  Illegal BsaI.rc site found at 5548
  Illegal BsaI.rc site found at 7833
  Illegal SapI.rc site found at 3328
  Illegal SapI.rc site found at 3874
  Illegal SapI.rc site found at 7725