Part:BBa_K957004

nah Operon for degradation of naphthalene to salicylate

One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1867
  Illegal PstI site found at 5862
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4122
  Illegal PstI site found at 1867
  Illegal PstI site found at 5862
  Illegal NotI site found at 8165
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 5215
  Illegal BamHI site found at 3200
  Illegal BamHI site found at 5902
  Illegal XhoI site found at 1311
  Illegal XhoI site found at 3699
  Illegal XhoI site found at 3851
  Illegal XhoI site found at 9353
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1867
  Illegal PstI site found at 5862
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1867
  Illegal PstI site found at 5862
  Illegal NgoMIV site found at 1804
  Illegal NgoMIV site found at 3579
  Illegal NgoMIV site found at 5453
  Illegal NgoMIV site found at 6065
  Illegal NgoMIV site found at 9378
  Illegal AgeI site found at 642
  Illegal AgeI site found at 3573
  Illegal AgeI site found at 6281
  Illegal AgeI site found at 8276
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2921
  Illegal BsaI site found at 3594
  Illegal BsaI site found at 4185
  Illegal BsaI site found at 5351
  Illegal BsaI.rc site found at 2755
  Illegal BsaI.rc site found at 5505
  Illegal BsaI.rc site found at 7790
  Illegal SapI.rc site found at 3285
  Illegal SapI.rc site found at 3831
  Illegal SapI.rc site found at 7682