Part:BBa_K953004
Hemeoxygenase with RBS (E. coli Codon Optimised)
Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953000 in that it does not includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (Part BBa_K953003). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.
The results and characterisation of our heme oxygenase/bacteriophtyochrome switch can be found here and you can also check out our entire project be clicking here.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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