Part:BBa_K953002

Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)

Agrobacterium tumefaciens bacteriophytochrome with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953003 in that it does not includes a T7 promoter (Part BBa_I712074). In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light (620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase with T7 promoter (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin.

The results and characterisation of bacteriophtyochrome can be found here and you can also check out our entire project be clicking here.

Visualisation of Agrobacterium tumefaciens Bacteriophytochrome, with correct bands approximately 2kb, thus determining the constructs were successful.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1513
  Illegal BamHI site found at 1873
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]