Part:BBa_K953001
Deinococcus radiodurans Bacteriophytochrome with RBS (E. coli Codon Optimised)
Deinococcus radiodurans bacteriophytochrome with a RBS and codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light (620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase with T7 promoter (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin.
The results and characterisation of bacteriophtyochrome can be found here and you can also check out our entire project be clicking here.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 919
Illegal BamHI site found at 724 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 337
Illegal NgoMIV site found at 1258
Illegal AgeI site found at 524
Illegal AgeI site found at 569
Illegal AgeI site found at 1630 - 1000COMPATIBLE WITH RFC[1000]
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