Intermediate

Part:BBa_K952005

Designed by: Saimon Sharif   Group: iGEM12_Columbia-Cooper-NYC   (2012-10-01)


IPTG inducible promoter-B0034-YF1-B0034-FixJ

This part has the IPTG inducible promoter with ribosome binding site (BBa_J04500) upstream and BBa_K592016 downstream. This will allow for YF1, blue-light sensing protein, and the response regulator, FixJ be promoted when IPTG is present. This can be used in conjunction with FixK2 (BBa_K592006) to selectively express when a cell is exposed to blue-light. This part is available with a constitute promoter in BBa_K952004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 970
    Illegal NgoMIV site found at 1482
    Illegal NgoMIV site found at 1775
    Illegal NgoMIV site found at 1869
    Illegal AgeI site found at 504
    Illegal AgeI site found at 1650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1539
    Illegal BsaI.rc site found at 403


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