Intermediate

Part:BBa_K952002

Designed by: Saimon Sharif   Group: iGEM12_Columbia-Cooper-NYC   (2012-10-01)

B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette

This part has BBa_K952001 downstream and BBa_K592016 upstream. This part causes cells to become blue-light sensitive; the cells will lyse when exposed to blue-light. This part is available with a constitutive promoter in BBa_K952006 and an IPTG inducible promoter BBa_K952007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 276
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311
    Illegal BsaI.rc site found at 175


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Categories
Parameters
None