Coding
Part:BBa_K945007
Designed by: Daniel Villalobos Ramírez Group: iGEM12_Tec-Monterrey_EKAM (2012-09-27)
Pichia pastoris optimized His-CrtB-PTS1
This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. It also includes a yeast Kozak consensus sequence and the AOX1 transcription terminator sequence for its use in expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1529
Illegal XbaI site found at 1558
Illegal PstI site found at 1342 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1529
Illegal PstI site found at 1342 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1529
Illegal BamHI site found at 1333
Illegal BamHI site found at 1364 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1529
Illegal XbaI site found at 1558
Illegal PstI site found at 1342 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1529
Illegal XbaI site found at 1558
Illegal PstI site found at 1342
Illegal AgeI site found at 1052 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 976
Illegal BsaI.rc site found at 1316
Illegal SapI site found at 14
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Categories
Parameters
None |