Composite

Part:BBa_K944013

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


Transcriptional Regulator and cioAB expression platform

This is the expression platform for our brand new biobricks. We want both the cioAB gene complex to express constitutively so the bacteria, in this case E. coli, can resist to diferent levels of cyanide. We also want the transcriptional regulator to be constitutive because it will interact with the cyanide to further activate activate the inducible promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2441
    Illegal XhoI site found at 2329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal NgoMIV site found at 1427
    Illegal NgoMIV site found at 1779
    Illegal NgoMIV site found at 2754
    Illegal NgoMIV site found at 2995
    Illegal NgoMIV site found at 3136
    Illegal NgoMIV site found at 3378
    Illegal AgeI site found at 1735
    Illegal AgeI site found at 2287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 560
    Illegal BsaI.rc site found at 3343
    Illegal BsaI.rc site found at 3652
    Illegal BsaI.rc site found at 5138
    Illegal SapI.rc site found at 3418


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