Intermediate

Part:BBa_K944012

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


T7 constitutive promoter with RBS, transcriptional regulator from de cyanide inducible promoter and

This BioBrick is the ligation of the biobrick BBa_K944009 and the biobrick BBa_K944010 which is an intermediate part to form our composite part which aims to express the cioAB cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal NgoMIV site found at 1427
    Illegal NgoMIV site found at 1779
    Illegal AgeI site found at 1735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 560


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Categories
Parameters
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