Intermediate

Part:BBa_K944006

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


CioAB genes - Cyanide-Insensitive terminal oxidase

CioAB has an important role in allowing aerobic respiration under cyanogenic growth conditions, because it encodes the subunits of a terminal oxidase homologous to the cytochrome bd quinol oxidases in bacteria. Under low oxygen tensions, these proteins provide an alternative to electrons transport.

We used this part to give resistance to cyanide to our bacteria under low oxygen tension.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 434
    Illegal XhoI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 747
    Illegal NgoMIV site found at 988
    Illegal NgoMIV site found at 1129
    Illegal NgoMIV site found at 1371
    Illegal NgoMIV site found at 1393
    Illegal NgoMIV site found at 1797
    Illegal NgoMIV site found at 1918
    Illegal AgeI site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1336
    Illegal BsaI.rc site found at 1645
    Illegal SapI.rc site found at 1411


[edit]
Categories
Parameters
None