Part:BBa_K942009
SS+ Zea m 14+His
In its native organism, Zea mays, Zea m 14 works as a lipid-transfer protein. It is known for causing allergenic response in humans. The secretion signal (SS) from α-mating factor pre-sequence is included for this construct also for extracellular secretion. (Gomar J., 1996) This protein has an estimated molecular weight of 9.8 kDa and is better visualized in a SDS-PAGE with Tris-Tricine gel buffer, because of its low molecular weight (Gasteiger E., 2005). It can be purified easily by affinity purification taking advantage of the 6xHis tag properties.
About Safety The Zea m 14 protein is a major food allergen from the maize, so any contact with cells transformed with this part should be done with appropriate gloves. Also we recommend that the use must make sure he/she is not allergic to maize. The secretion of this protein makes this part more hazardous so you would like to have extra precaution to prevent any leak from your samples
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 194
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