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Part:BBa_K940000

Designed by: Louise Usher   Group: iGEM12_Westminster   (2012-09-25)


ALDH1A1 promoter in a pSB1C3 backbone

This part is a promoter for ALDH1A1,an enzyme involved in alcohol metabolism. Nucleotide sequences were analysed on NCBI. Sequences were selected by determining the start of the protein coding region and then taking the previous 1000 bases which included the TAATAA box. The sequences selected were compared with commercial sequences. Additionally overlap between the selected sequence and sequences on the Eukaryotic Promoter database (EPD) was confirmed. ALDH sequence was selected to exclude BioBrick restriction sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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