Part:BBa_K936035

Strain E-15 EG3

Function: The strain is capable of utilizing ethylene glycol as a sole carbon source, and grows optimally at 30 mM of ethylene glycol media.
Note: The original stab culture was re-streaked on ethylene glycol agar for three generations to ensure degradation activity.

Media Components (per liter):

34 mM NaH₂PO₄

64 mM K₂HPO₄

20 mM (NH₄)₂SO₄

1 μM FeSO₄

0.1 mM MgSO₄

10 μM CaCl₂

30 mM Ethylene Glycol

For Agar plates (per liter):
Add in the above components and add 15g of bactoagar to achieve a 1.5% (w/v) mixture.

Growth Rates: (at 37°C, at 150rpm)

In 30 mM ethylene glycol media: around 24 hours to reach OD 0.2

In 30 mM ethylene glycol agar: around 84 hours to develop single colonies.

Experimental Results:



When Strain E-15 EG3 was repassaged in new media, we saw a general increase in growth rate over time. We performed two trials simultaneously on the same original strain, labeled Tube 1 and Tube 2. For Tube 1, we saw a 74.8% increase and for Tube 2, we saw a 227.84 % increase, both over a time period of 285.95 hours.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]