Device
2gen:ArsR

Part:BBa_K935004

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)

E. coli ArsR generator coupled to pArs-ArsR-RFP

This device has two coding sequences for ArsR under the control of pArs with the second pArs also controlling the expression of RFP. There is no scar between the second pArs and mRFP1 to allow reporter substitution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 918
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1762
    Illegal AgeI site found at 1874
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None