Device
tuned pArs

Part:BBa_K935002

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)

E. coli ArsR under the control of pArs coupled with pArs + RFP

This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter. This device was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction of RFP expression. pArs-RFP was acquired from BBaK190015 and therefor there is no scar between the promoter and reporter allowing for a reporter substitution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1316
    Illegal AgeI site found at 1428
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None