Generator
ArsR Gen.

Part:BBa_K935001

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)

E. coli chromosomal ars promoter with arsR repressor gene and double terminator (B0010-B0012)

This part consists of the composite part BBa_J33201 designed by the 2006 Edinburgh Team which contains the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (pArs), an RBS, and the gene for the ArsR repressor binding protein followed by an ORF. To create an ArsR generator under the control of the pArs we added a double terminator. This generator directs the production of ArsR at a basal transcription rate and this production will increase proportionally n the cell with an increase of a internal concentration of sodium arsenate or sodium arsenite (see also 2009 Gronigen for details: http://2009.igem.org/Team:Groningen/Modelling/Characterization)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None