Device

Part:BBa_K933004

Designed by: Chris Cetnar   Group: iGEM12_Penn_State   (2012-10-11)


Palindromic sequence #1 in directionality analysis construct

This part is a palindromic sequence designed to be bidirectional. The specific part has the directionality construct with palindrome #1 (BD#1) as the inserted promoter. By digesting at the restriction site xba1 and Spe1 this will remove the promoter sequence, one can then ligate in different promoters at the restriction sites to measure the directionality of different promoter sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None