Coding

Part:BBa_K933001

Designed by: Hannah Jepsen-Burger   Group: iGEM12_Penn_State   (2012-10-01)


leader sequence with threonine repeats. mCherry and GFP reporters

This part tests codon optimization of the theonine codons. It is prefaced by an AHL leader sequence, then a 6-codon repeat sequence of one codon, followed by an mCherry and GFP reporter. The reporters determine the amount of tRNA degradation which occurs as a result of the repeat sequence. Use to determine other codon optimization characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 66
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 66
    Illegal NheI site found at 94
    Illegal NheI site found at 117
    Illegal NotI site found at 72
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 66
    Illegal BamHI site found at 197
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 66
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 66
    Illegal XbaI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None