Reporter

Part:BBa_K933000

Designed by: Hannah Jepsen-Burger   Group: iGEM12_Penn_State   (2012-10-01)

two start codons coding for RFP and sfGFP reporters

This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested.



Fluorescencemsc.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 50
    Illegal SpeI site found at 1651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal NheI site found at 43
    Illegal SpeI site found at 1651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 98
    Illegal BamHI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 50
    Illegal SpeI site found at 1651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 50
    Illegal SpeI site found at 1651
    Illegal NgoMIV site found at 1484
    Illegal AgeI site found at 638
    Illegal AgeI site found at 750
    Illegal AgeI site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//terminator/double
Parameters
None