Intermediate

Part:BBa_K931007

Designed by: Jasmine Jiang   Group: iGEM12_Nevada   (2012-09-28)

SBP - B12BP

A construct which contains a starch binding domain encoded by the gene, CBM 21 and the BtuF gene that ecodes for a B12 binding protein, with B12BP on the N-Terminus. The BtuF domain is from the gene, BtuCDF, involved in vitamin B12 transfer into E. coli’s periplasmic region. The CBM 21 domain is part of the amylolytic enzyme family utilized by organisms involved in carbohydrate degradation. When expressed in concert, this construct bridges vitamin B12 to a starch substrate. The genes encoding for the B12 binding and starch binding domains come from the parts (BBa_K931001) and (BBa_K931000), respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 691
    Illegal PstI site found at 705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 691
    Illegal PstI site found at 705
    Illegal NotI site found at 7
    Illegal NotI site found at 698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 691
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 691
    Illegal PstI site found at 705
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None