Coding

Part:BBa_K931004

Designed by: Jeremiah Pavelka   Group: iGEM12_Nevada   (2012-10-01)

Thiamine Binding Protein (TBP)

The Thiamine-Binding Protein is coded for by a 981bpgene (THiT) that comes from the E. coli bacteria. The membrane thiamine-binding protein is optimized for E. coli and has a gene ID of 88096. Thiamine-binding protein plays a crucial role in the thiamine transport system by binding the thiamine molecule. It binds thiamine at a Kd of 170 nM when the optimum pH is at 5.0. The thiamine-binding protein also transports thiamine with a Km of 180 nM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 867
    Illegal NgoMIV site found at 894
    Illegal AgeI site found at 57
    Illegal AgeI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None