Part:BBa_K931003

An L-Arabinose Controlled Vitamin B12 Binding - Starch Binding Protein Fusion

L-arabinose induced Starch Binding-B12 Binding Protein is a functional construct which contains the BtuF gene that ecodes for a B12 binding protein and a starch binding domain encoded by the gene, CBM 21. The construct is under the control of an L-arabinose promoter (BBa_K206000) and a terminator (BBa_K206000). The BtuF domain is from the gene, BtuCDF, involved in vitamin B12 transfer into E. coli’s periplasmic region. The CBM 21 domain is part of the amylolytic enzyme family utilized by organisms involved in carbohydrate degradation. When expressed in concert, this construct bridges vitamin B12 to a starch substrate. The genes encoding for the B12 binding and starch binding domains come from the parts (BBa_K931001) and (BBa_K931000), respectively.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 776
  Illegal XbaI site found at 791
  Illegal SpeI site found at 1118
  Illegal PstI site found at 1132
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 776
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 1118
  Illegal PstI site found at 1132
  Illegal NotI site found at 782
  Illegal NotI site found at 1125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 776
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 776
  Illegal suffix found in sequence at 1118
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 776
  Illegal XbaI site found at 791
  Illegal SpeI site found at 1118
  Illegal PstI site found at 1132
  Illegal AgeI site found at 616
  Illegal AgeI site found at 728
  
• 1000
  COMPATIBLE WITH RFC[1000]