Coding

Part:BBa_K931000

Designed by: Joe Alexander   Group: iGEM12_Nevada   (2012-09-27)

Starch Binding Protein (SBP)

Starch Binding Protein (SBP), also known as Starch Binding Domain, is a protein domain from the gene CBM 21, which codes for the two domain glucoamylase enzyme in the organism Rhizopus oryzae. Its functional purpose is to aid starch breakdown by binding to starch and providing an optimal interaction with the amylytic enzymes. Its substrates are diverse among the carbohydrate family. It has a high affinity for rice starch, corn starch, sweet potato starch, tapioca starch, and also wheat starch. from . This starch binding domain can function as a protein purification tag much like the MBP, but using a much less expensive starch purification column.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
    Illegal NotI site found at 7
    Illegal NotI site found at 350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 343
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None