Part:BBa_K925007

MyToxic

This part was designed to bind toxic metals; cadmium, mercury and cobalt. This was done by inserting flexible linkers between the metal binding sites. The linkers were used because they were quite flexible and prevented hairpins in the structure.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 195
  Illegal PstI site found at 414
  Illegal PstI site found at 637
  Illegal PstI site found at 856
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 195
  Illegal PstI site found at 414
  Illegal PstI site found at 637
  Illegal PstI site found at 856
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 105
  Illegal BamHI site found at 547
  Illegal XhoI site found at 441
  Illegal XhoI site found at 883
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 195
  Illegal PstI site found at 414
  Illegal PstI site found at 637
  Illegal PstI site found at 856
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 195
  Illegal PstI site found at 414
  Illegal PstI site found at 637
  Illegal PstI site found at 856
  
• 1000
  COMPATIBLE WITH RFC[1000]

Description

The aim of this part was to produce a protein that would act as a general metal scavenger. This protein was designed to bind to toxic metals; cadmium, mercury and cobalt. This was done by inserting flexible linkers between the metal binding sites. This prevented hairpins in the structure stopping binding site function.

Characterisation

No characterisation has been done.

Results

No results have been found.

Conclusion

This part has been succesfully ligated into a standard iGEM vector but no expression or characterisation work has been done.