Coding

Part:BBa_K918001

Designed by: steven denham   Group: iGEM12_Colorado_State   (2012-09-29)


Kumamolisin-As_N291D for yeast

Kumamolisin-As_N291D, designed by the 2011 University of Washington team to break down gluten, with codons altered for optimal expression in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1231
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 421
    Illegal BglII site found at 556
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1231
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 530


[edit]
Categories
Parameters
None