Translational_Unit

Part:BBa_K917007

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-08-10)


S. oneidensis decaheme cytochrome c electron export system mtrCAB

This part consists of three genes from the native gene cluster from Shewanella oneidensis MR-1 with native RBSs: MtrA is a decaheme cytochrome c located between inner and outer membrane; MtrB is aa transmembrane beta-barrel responsible for facilitating contact between MtrA and MtrC; MtrC (alternative name: OmcB) is a decaheme cytochrome c attached to the outer membrane. Together these proteins are able to form an extracellular electron export system. (Jensen et al, 2010, PNAS vol. 107 no. 45 19213-19218; doi: 10.1073/pnas.1009645107)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 275


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Categories
Parameters
None