Composite

Part:BBa_K917005

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-20)

lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae


This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of metronidazole leads to growth inhibition (see Experience page). This gene can be used as a counter selectable marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1189
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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