Translational_Unit

Part:BBa_K917004

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-20)

nfsI nitroreductase gene from Enterobacter cloacae

This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductase. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of metronidazole leads to growth inhibition (see Experience page). This gene could possibly could be counter selectable marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 581
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None