Part:BBa_K916003
Leucine Zipper NGFP translational fusion
This is a leucine zipper domain fused to the N terminal fragment of GFP used in GFP fragment implementation assays. This part has been sequenced and is fully characterized. Flow cytometry data showing it's ability to produce fluorescence can be found both below and [http://2012.igem.org/Team:Georgia_Tech on our wiki].
A paper showing the source of this part can be found in our references section
NOTE: This part has no promoter, and so can be used with any BioBrick promoter in the registry. See the list of promoters here.
Usage and Biology
This is for use with part BBa_K916004 as a [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation GFP-fragment complementation] based biosensor.
This part codes for a fusion protein of the N-terminal fragment of GFP (NGFP) and a [http://en.wikipedia.org/wiki/Leucine_zipper leucine zipper domain.] Leucine zipper domains form homodimers. When this protein is expressed in a cell also expressing the leucine zipper CGFP fusion (encoded in part BBa_K916004), the dimerization brings the GFP fragments into proximity and allows them to interact and fluoresce like an intact GFP peptide.
This is flow cytometry data showing a negative control with no induction of the GFP fragments, showing a population distribution in the range of no flourescence
This is flow cytometry data showing that, in a cell also expressing BBa_K916004, this part produces fluorescence population-wide
Fluorescent colonies were only observed on plates when both this part and BBa_K916004 were induced in the cells, as is the case on the bottom right plate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 618
Illegal XhoI site found at 521 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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