Part:BBa_K916000

TraR Coding Sequence

TraR Coding Sequence from Agrobacterium tumefaciens

NOTE: This part has no promoter, and so can be used with any BioBrick promoter in the registry. See the list of promoters here.

Usage and Biology

3-oxo-C8-HSL Figure 1 Luo ZQ, Su S, Farrand SK. In situ activation of the quorum-sensing transcription factor TraR by cognate and noncognate acyl-homoserine lactone ligands: kinetics and consequences. J Bacteriol. 2003 Oct;185(19):5665-72. PMID: 13129937 http://www.ncbi.nlm.nih.gov/pubmed/13129937

TraR is the A. tumefaciens transcriptional regulator responsible for its [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] response. It binds N-(3-Oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL), and upon binding forms a homodimer. Most similar [http://en.wikipedia.org/wiki/LuxR-type_DNA-binding_HTH_domain LuxR family] quorum sensing regulators dimerize without their cognate autoinducer, but TraR will only dimerize upon binding 3-oxo-C8-HSL.

However, it has been shown that TraR cannot activate transcription in E. coli without adding other accessory factors from Agrobacterium. [http://www.ncbi.nlm.nih.gov/pubmed/19732344 SOURCE.]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]