RNA

Part:BBa_K914000

Designed by: Jean Cury   Group: iGEM12_Paris_Bettencourt   (2012-09-18)

pLac-supD-T

This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here). This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.


This part has been improved by part BBa_K2725016 - supD expressed under J23108 anderson promotor (strength 0.51). This expression level of supD allows the expression of a gene with 5 serine codons substituted for amber STOP codons.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99


[edit]
Categories
//biosafety/semantic_containment
Parameters
None