Composite

Part:BBa_K912030

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-03)


pH Inducible Lysis System(w)

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2609
    Illegal NheI site found at 2632
    Illegal NheI site found at 3059
    Illegal NheI site found at 3082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2214
    Illegal AgeI site found at 2284
    Illegal AgeI site found at 3631
    Illegal AgeI site found at 4375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2865


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None