Composite

Part:BBa_K912029

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-03)


Pgad/lysis

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high intracellular chloride concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2609
    Illegal NheI site found at 2632
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2214
    Illegal AgeI site found at 2284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2865


[edit]
Categories
Parameters
None