Composite

Part:BBa_K912028

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23114(weak) promoter/RBS+ C. Braakii phytase

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to constitutively produce c. braakii phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1046
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 108


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Categories
Parameters
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