Composite

Part:BBa_K912023

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23104(strong) promoter/RBS+ E. coli phytase

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to constitutively produce e. coli phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 729
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1254


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Categories
Parameters
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