Composite

Part:BBa_K912015

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23114(weak) Constitutive promoter + ClC-ec1 chloride antiporter

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to constitutively produce CLC-EC1, a chloride-hydrogen antiporter that pumps protons out of the cell and chloride ions into the cell in acidic conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 1323
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None