Composite

Part:BBa_K912007

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-09-28)


Pgad/GFP composite part

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to fluoresce in the presence of high chloride concentrations. The Pgad promoter in front of the GFP is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1901


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