Part:BBa_K911003
Fluoride Sensitive Riboswitch
Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch (the crcB gene) knocked out (kindly provided by the Breaker lab in Yale). Characterisation data can be found in the experience tab of this part.
Usage and Biology
This riboswitch is a positive regulator. The riboswitch, originally isolated from Bacillus cereus, serves as a transcriptional attenuator in the absence of fluoride. In the presence of fluoride its conformation changes and the repression is lifted. In B. cereus this serves to permit translation of a fluoride efflux pump, which allows the bacteria to cope with the potentially toxic fluoride levels in which it finds itself.
We have found, results in the experience tab, that the linear region of this riboswitch is in the 0uM to 100uM range when using β-galactosidase as the reporter in a Miller assay, and in the 1mM to 30mM range when using β-galactosidase as the reporter in an X-Gal assay.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 26
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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