Regulatory

Part:BBa_K909016

Designed by: Sandro Kundert   Group: iGEM12_ETH_Zurich   (2012-09-24)

PR promoter with two operator sites: cI OR1, cI OR2 and and LacI O1/O2

The promoter is derived from the PR (lambda phage) promoter. The -35 and -10 regions as well as the cI operator sites are maintained while the sequence downstream of the -10 region is replaced by the LacI operator LacO1 and LacO2.

This part serves as a biological NOR-gate

Laci ci.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None