Composite

Part:BBa_K909015

Designed by: Deborah Huber   Group: iGEM12_ETH_Zurich   (2012-09-22)

p-ABA overproduction composite without promoter

p-ABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of p-ABA. ADC synthase is composed of two subunits, PabA and PabB/C. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates p-ABA.

pABA is synthesized from chorismate in a 2-step process unsing pabA, pabB and pabC. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855612/ Wegkamp et al.]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None