Composite

Part:BBa_K909014

Designed by: Deborah Huber   Group: iGEM12_ETH_Zurich   (2012-09-22)

Constitutive expression of pABA

PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA. ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.


pABA is synthesized from chorismate in a 2-step process unsing pabA, pabB and pabC. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855612/ Wegkamp et al.]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None