Regulatory

Part:BBa_K909011

Designed by: Deborah Huber   Group: iGEM12_ETH_Zurich   (2012-09-23)

Dual input promoter: PL promoter with two operator sites TetO1 and LacO1

The promoter is derived from the PL (lambda phage) promoter. The -35 and -10 regions are conserved while the cI operator sites and sequence between the -35 and -10 regions was replaced by the operator regions TetO1 from the tetR promoter on Transposon Tn10 and the LacI operator regions LacO1 and LacO2.

This part serves as a biological NOR-gate.

Tetlac.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None