Deleted
Sample Discontinued
Experience: None
No Sequence
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Plasmid_Backbone

Part:BBa_K906201

Designed by: Bert Berla   Group: iGEM12_WashU   (2012-08-14)


Psl2131 (for Synechocystis homologous recombination)

Kan/Amp resistant plasmid for Homogous recombination into Synechocystis 6803. Please note that this plasmid is only biobrick friendly and not compatible due to an EcoRI site present above the upstream homology region. To use this with biobrick compatible parts, assembly should be completed in a standard, highcopy plasmid such as Psb1C3 and then inserted into this backbone for transformation into Synechocystis using XbaI and PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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