Part:BBa_K906102

ZCD with Histidine Patch Thioredoxins and Hist-tag

This is the functional unit which makes ZCD soluble in E. coli. It includes a inducible promoter, mutated thioredoxin to increase solubility, the ZCD ORF, a hist-tag for easy purification and finally a double terminator. This part has been shown to highly express ZCD in E. coli. It is suggested that this part be used in a plasmid also containing araC for the optimal repression of this protein before the time of induction.

The information that lead to the development of the this vector tag with ZCD came out of the following paper:
Bouvier, Florence et al. “Oxidative Remodeling of Chromoplast Carotenoids : Identification of the Carotenoid Dioxygenase CsCCD and CsZCD Genes Involved in Crocus Secondary Metabolite Biogenesis.” 15.January (2003): 47–62.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 239
  Illegal BamHI site found at 1452
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 673
  Illegal AgeI site found at 74
  Illegal AgeI site found at 1849
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1831
  Illegal BsaI site found at 1884
  Illegal BsaI site found at 2062
  Illegal SapI site found at 56